To address the demand for mild and specific purification, a protein purification ligand with calcium-dependent binding to IgG has been developed. .. Read more Further improvement of this concept will be presented, including a novel protocol for antibody purification, as well as the development of new tailor-made ligands for purification of other biological therapeutics. Nanobodies are highly versatile tools for protein purification and detection. Here, recent advances on how generic nanobodies, in combination with novel tagging strategies, can be applied for protein purification, and can monitor protein levels in real time will be presented. Over the last few decades, several notable innovations have accelerated research and simplified manufacturing of new therapeutic proteins. Currently under consideration is the use of self-cleaving tag methods to streamline discovery through commercialization. Affinity adsorbents have been the cornerstone in protein purification.
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