A novel automated and simultaneous protein/antibody high-throughput purification platform: AmMag and its reusable Magbeads

High throughput purification is a critical step for antibody engineering and screening. Protein purification using traditional resin columns is .. Read more limited by throughput and a complicated sample preparation process, which makes it challenging to extend to high throughput antibody purification. Magnetic beads-based purification systems are simpler to operate as they eliminate the need to remove cells by centrifugation and sample filtration, and are easily applied to high throughput purification while providing significant savings in time and labor. Here we present novel solutions, such as the AmMag (TM) SA semi-automatic purification device and AmMag (TM) Ni beads protein A purification beads that enable high-throughput antibody purification using magnetic beads. The tools and techniques described here, are expected to significantly speed up antibody purification and screening while increasing cost-effectiveness.