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Light-sheet fluorescence microscopy (LSFM) has generated significant interest in the biological community. Not only does it deliver light to only the in-focus portion of a specimen, decreasing the illumination burden, but the resulting fluorescence can be collected with modern scientific cameras, allowing for millionfold detection parallelization. Nevertheless, despite these advantages,
...
its adoption for subcellular imaging remains limited.
The reasons for this include complicated sample preparation, the reliance on high-NA water-dipping objectives, and the lack of modalities that make microscopy routinely useful, such as sample environment control and laser-based autofocusing. There is, however, one form of LSFM, referred to as oblique plane microscopy, that avoids these complications and is compatible with traditional sample mounting, environment control, and autofocusing mechanisms.
The reasons for this include complicated sample preparation, the reliance on high-NA water-dipping objectives, and the lack of modalities that make microscopy routinely useful, such as sample environment control and laser-based autofocusing. There is, however, one form of LSFM, referred to as oblique plane microscopy, that avoids these complications and is compatible with traditional sample mounting, environment control, and autofocusing mechanisms.
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Estimated Turnout100 - 500 Delegates Based on previous editions | Event TypeConference |
EditionsAug 2020Frequency Not Available | Official LinksWebsiteContacts Report Error Claim this event |
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