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11 May 2021Ended

How Immunohistochemistry and Immunofluorescence Benefit Pre-Clinical and Clinical Research

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Immunohistochemistry (IHC) and immunofluorescence (IF) are molecular assays that involve the use of antibodies to detect specific proteins within .. Read more tissues on microscope slides. Unlike other antibody assays, immunohistochemistry allows researchers to visualize exactly where proteins are localized within tissues (specific cell types and subcellular localization; cell membrane, cytoplasm or nuclear). Providing cellular identity within the context of the tissue allows pathologists to identify which cells within that tissue are expressing the protein. Additional markers may be used to further identify tumor origin and markers such as Ki67 or PCNA, which can be used to determine how rapidly neoplastic cells are proliferating. Immunohistochemistry can also be used to distinguish apoptosis from necrosis and endothelial cell markers, such as CD31, can be used to evaluate tumor angiogenesis. StageBio is a leading provider of GLP-compliant necropsy, histology, pathology, and specimen archiving services for the biopharmaceutical, medical device, academic, and contract research industries. Our hand-curated daily news feed and active message forums will keep you informed.

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