In this presentation, scientists from Regeneron are going to discuss two emerging LC-MS based methods for characterization of AAV capsids under both .. Read more denatured and near native states. To characterize the constituent capsid proteins, hydrophilic interaction chromatography, operated under denaturing conditions, can be coupled with mass spectrometry (HILIC-MS). This method was successfully applied to characterize a wide variety of AAV serotypes to achieve unambiguous serotype identification, PTM characterization, and stoichiometry assessment. In addition, they will also discuss the development and application of a rapid online native MS method to assess and potentially quantify empty, partially- and fully-filled capsids.
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